ON THE CONSTRUCTING OF SURVIVAL CURVES FOR CULTURED CELLS IN CYTOGERONTOLOGICAL EXPERIMENTS: A BRIEF NOTE WITH THREE HIERARCHY DIAGRAMS
Abstract
A brief comparative analysis of different approaches to cell viability testing in cytogerontological experiments is made. Particular emphasis is placed upon problems arising under constructing of survival curves for cultured cells in the “stationary phase aging” model. It is noted that the choice of the appropriate methods is mainly determined by the researchers‘ ideas about molecular and cellular mechanisms of aging. It is emphasized that the evaluation of cell cloning efficiency, though optimal for cell viability determination, is, unfortunately, nonapplicable to postmitotic or very slowly propagating cells. Some problems encountered when using the most popular molecular probes designed for “live/dead” assays of cell population are also briefly reviewed.
About the Authors
A. N. Khokhlov
биологический факультет МГУ
Russian Federation
Russian Federation
G. V. Morgunova
биологический факультет МГУ
Russian Federation
Russian Federation
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For citations:
Khokhlov A.N., Morgunova G.V. ON THE CONSTRUCTING OF SURVIVAL CURVES FOR CULTURED CELLS IN CYTOGERONTOLOGICAL EXPERIMENTS: A BRIEF NOTE WITH THREE HIERARCHY DIAGRAMS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2015;(2):19-23. (In Russ.)
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