THE EFFECT OF SHORT-TERM HYPOXIC STRESS ON IMMUNOSUPPRESSIVE ACTIVITY OF PERIVASCULAR MULTIPOTENT STROMAL CELLS
Abstract
Multipotent mesenchymal stromal cells (MSCs) are stromal precursors with capacity to differentiate in osteo-, adipo-, and chondrodirections, participate in repair, regeneration and immune response. Those abilities, especially immunosuppression, make MSCs a perspective tool for cell therapy and regenerative medicine. Short-term hypoxic stress can occur in damaged tissues and negatively affect MSC capacities to modulate functions of activated peripheral blood mononuclear cells (PBMCs). In present paper, the impact of short-term hypoxic stress (<1% of oxygen) on immunosuppressive potential of tissue oxygen (5%) adapted MSCs was evaluated. At tissue oxygen level, we detected an increase of the ratio of innate immune cells (natural killers, NK) and a decrease of the ratio of adaptive immune cells (HLA-DR+ Т-cells) within floating PBMCs in the presence of MSCs. Additionally, inhibition of T-cell proliferation was observed.
Within adhered PBMCs the ratio of monocytes was higher and the ratio of NK-T-cells was lower.
Short-term hypoxic stress did not affect MSC immunosuppression toward lymphocytes in suspension.
Nevertheless, a decrease of percent of monocytes and NK-T-cells within adhered PBMCs was detected. Thus, hypoxic stress did not influence immunosuppressive activity of MSCs toward floating PBMCs. Attenuation of monocyte adhesion to MSCs upon cell-to-cell interaction may negatively impact on development of MSC-educated macrophages phenotype with anti-inflammatory activity. In vivo it may provoke slowdown of “response to injury” during inflammation.
About the Authors
K. V. Zornikova
Institute of Biomedical Problems, Russian Academy of Sciences;
Lomonosov Moscow State University
Russian Federation
Russian Federation
Horoshevskoye sh. 76A, Moscow, 123007, Russia;
Leninskiye gory 1–12, Moscow, 119234, Russia
Department of Cell Biology and Histology, Faculty of Biology
A. N. Gornostaeva
Institute of Biomedical Problems, Russian Academy of Sciences;
Russian Federation
Horoshevskoye sh. 76A, Moscow, 123007, Russia;
Russian Federation
Horoshevskoye sh. 76A, Moscow, 123007, Russia;
E. R. Andreeva
Institute of Biomedical Problems, Russian Academy of Sciences;
Russian Federation
Horoshevskoye sh. 76A, Moscow, 123007, Russia;
Russian Federation
Horoshevskoye sh. 76A, Moscow, 123007, Russia;
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Review
For citations:
Zornikova K.V., Gornostaeva A.N., Andreeva E.R. THE EFFECT OF SHORT-TERM HYPOXIC STRESS ON IMMUNOSUPPRESSIVE ACTIVITY OF PERIVASCULAR MULTIPOTENT STROMAL CELLS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2018;73(1):16-21. (In Russ.)
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