FLUORESCENCE OF WHITE SEA PHYTOPLANKTON UNDER DIFFERENT NITROGEN SOURCE AND TWO LEVELS OF IRRADIANCE
https://doi.org/10.1234/XXXX-XXXX-2012-3-33-38
Abstract
The response of phytoplankton fluorescence and biomass to addition of different nitrogen sources and irradiance were assayed in enriched bottle experiments with the White Sea phytoplank ton in August—September 2007. Phytoplankton was exposed in situ 18 days with the additions of 180 mM/L of nitrogen as nitrate, urea, ammonium and glycine under two levels of irradiance. The maximum quantum efficiency of PSII (Fv/Fm) was determined in the dark-acclimated algae. Rapid light curves (RLC) were constructed based on 8 actinic increasing light levels. The maximal relative electron transport rate (rETRmax), the maximum light use coefficient (a), and the non-photochemical quenching (NPQ) were calculated. After enrichment abundance of phytoplankton increased, and the photosynthetic parameters changed. The maximum quantum efficiency of PSII increased to 0,64—0,71, indicating a good physiological state of algae and a lack of stress due to nutrient limitation. The dynamic of rETRmax and NPQ depended on nitrogen source and growth irradiances while a did not depended on nitrogen form.
About the Authors
L. V. IlyashRussian Federation
T. A. Belevich
Russian Federation
D. N. Matorin
Russian Federation
References
1. Glibert P.M. Primary productivity and pelagic nitrogen cycling // Nitrogen cycling in coastal marine environments / Eds. T.H. Blackburn, J. Sorensen. New York, 1988. P. 3—31.
2. Максимова М.П. Гидрохимия Белого моря // Гидрометеорология и гидрохимия морей СССР. 1991. Т. 2. Белое море. Ч. 1. С. 8—193.
3. Ильяш Л.В., Житина Л.С., Федоров В.Д. Фитопланктон Белого моря. М.: Янус-К, 2003. 168 c.
4. Falkowski P.G., Raven J.A. Aquatic photosynthesis. Malden: Blackwell Science, 1997. 375 p.
5. Antia N.J., Harrison J.P., Oliveira L. The role of dissolved organic nitrogen in phytoplankton nutrition, cell biology and ecology // Phycologia. 1991. Vol. 30. P. 1—89.
6. Mulholland M.R., Lee C., Glibert P.M. Extracellular enzyme activity and uptake of carbon and nitrogen along an estuarine salinity and nutrient gradient // Mar. Ecol. Prog. Ser. 2003. Vol. 258. P. 3—17.
7. Andersson M.G.I., van Rijswijk P., Middelburg J.J. Uptake of dissolved inorganic nitrogen, urea and amino acids in the Scheldt estuary: comparison of organic carbon and nitrogen uptake // Aquat Microb. Ecol. 2006. Vol. 44. P. 303—315.
8. Seitzinger S.P., Sanders R.W. Atmospheric input of dissolved organic nitrogen stimulate estuarine bacteria and phytoplankton // Limnol. Oceanogr. 1999. Vol. 44. P. 721—736.
9. Guillard R.R.L., Ryther J.H. Studies on marine diatoms. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. // Can. J. Microbiol. 1962. N 8. P. 229—239.
10. Ryther J., Dunstan W.M. Nitrogen, phosphorus and eutrophication in the coastal marine environment // Science. 1971. Vol. 171. P. 1008—1013.
11. Schreiber U. Pulse-Amplitude-Modulation (PAM) fluorometry and saturation pulse method: an overview // Chlorophyll a fluorescence: a signature of photosynthesis / Eds. G.C. Papageorgiou, Godvindjee. Dordrecht: Kluwer Academic Publishers, 2004. P. 279—319.
12. Jassby A.D., Platt T. Mathematical formulation of the relationship between photosynthesis and light for phytoplankton // Limnol. Oceanogr. 1976. Vol. 21. P. 540—547.
13. Hillebrand H., Durselen C.D., Kirschtel D., Pollingher U., Zohary T. Biovolume calculation for pelagic and benthic microalgae // J. Phycol. 1999. Vol. 35. P. 403—424.
14. Menden-Deuer S., Lessard D.J. Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton // Limnol. Oceanogr. 2000. Vol. 45. P. 569—579.
15. Parkhill J.-P., Maillet G., Cullen J.J. Fluorescencebased maximal quantum yield for PSII as a diagnostic of nutrient stress // J. Phycol. 2001. Vol. 37. P. 517—529.
16. Ильяш Л.В., Белевич Т.А., Уланова А.Ю., Маторин Д.Н. Флуоресцентные параметры морских планктонных водорослей при ассимиляции органического азота // Вестн. Моск. ун-та. Сер. 16. Биология. 2007. № 3. С. 17—22.
17. Kolber Z., Zehr J., Falkowski P.G. Effects of growth irradiance and nitrogen limitation on photosynthetic energy conversion in photosystem II // Plant Physiol. 1988. Vol. 88. P. 923—929.
18. Geider R.J., Roche J., Greene R., Olaizola M. Response of the photosynthetic apparatus of Phaeodactylum tricornutum to nitrate, phosphate, or iron starvation // J. Phycol. 1993. Vol. 29. P. 755—766.
19. Lippemeier S., Hintze R., Vanselow K.H., Harting P., Colijn F. In-line recording of PAM fluorescence of phytoplankton as a new tool for studying effects of fluctuating nutrient supply on photosynthesis // Eur. J. Phycol. 2001. Vol. 36. P. 89—100.
20. Young E.B., Beardall J. Rapid ammonium- and nitrate-induced perturbations to chlorophyll a fluorescence in nitrogen-stressed Dunaliella tertiolecta (Chlorophyta) // J. Phycol. 2003. Vol. 39. P. 332—342.
21. Young E.B., Beardall J. Photosynthetic function in Dunaliella tertiolecta (Chlorophyta) during a nitrogen starvation and recovery cycle // J. Phycol. 2003. Vol. 39. P. 897—905.
22. Fan C., Glibert P.M., Lomas M.W. Characterization of urease activity in three marine phytoplankton species, Aureococcus anophagerefferens, Prorocentrum minimum, and Thalassiosira weissflogii // Mar. Biol. 2003. Vol. 142. P. 949—958.
23. Levasseur M., Thompson P.A., Harrison P.J. Physiological acclimation of marine phytoplankton to different nitrogen sources // J. Phycol. 1993. Vol. 29. P. 587—595.
24. Blanchard G., Guarini J.-M., Dang C., Richard P. Characterizing and quantifying photoinhibition in intertidal microphytobenthos // J. Phycol. 2004. Vol. 40. P. 692—696.
25. Han B.-P., Virtanen M., Koponen J., Straskraba M. Effect of photoinhibition on algal photosynthesis: a dynamic model // J. Plankton Res. 2000.Vol. 22. P. 865—885.
26. Lomas M.W., Glibert P.M. Temperature regulation of nitrate uptake: a novel hypothesis about nitrate uptake and reduction in cool-water diatoms // Limnol. Oceanogr. 1999. Vol. 44. P. 556—572.
27. Muhlstein H.I., Villareal T.A. Organic and inorganic nutrient effects on growth rate-irradiance relationships in the texas brown-tide alga Aureoumbra lagunensis (Pelagophyceae) // J. Phycol. 2007. Vol. 43(6). P. 1223—1226.
28. Arsalane W., Rousseau B., Duval J.-C. Influence of the pool size of the xanthophyll cycle on the effects of light stress in a diatom: competition between photoprotection and photoinhibition // Photochem. Photobiol. 1994. Vol. 60. P. 237—243.
29. Lavaud J., Rousseau B., Etienne A.-L. Enrichment of the lightharvesting complex in diadinoxanthin and implications for the non-photochemical fluorescence quenching in diatoms // Biochemistry. 2003. Vol. 42. P. 5802—5808.
30. Lavaud J., Rousseau B., Etienne A.-L. General features of photoprotection by energy dissipation in planktonic diatoms (Bacillariophyceae) // J. Phycol. 2004. Vol. 40. P. 130—137.
31. Perkins R.G., Mouget J.-L., Lefebvre S., Lavaud J. Light response curve methodology and possible implications in the application of chlorophyll fluorescence to benthic diatoms // Mar. Biol. 2006. Vol. 149. P. 703—712.
Review
For citations:
Ilyash L.V., Belevich T.A., Matorin D.N. FLUORESCENCE OF WHITE SEA PHYTOPLANKTON UNDER DIFFERENT NITROGEN SOURCE AND TWO LEVELS OF IRRADIANCE. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2012;(3):33-38. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2012-3-33-38