Possibility of application of extracellular protease of micromycet Aspergillus ochraceus for determination of factor X content in human blood plasma
Abstract
It has been shown that activity of factor X activity determined in normal plasma using Aspergillus ochraceus protease is comparable with the activity of a commercial analogue, a protease from Russell’s viper venom (RVV-X®). It was revealed that the protease of A. ochraceus along with the RVV-X® preparation can be used to determine the content of factor X in the plasma with its reduced content. A study of the protease activity of A. ochraceus, an activator to factor X, showed that it is slightly higher compared to the snake venom preparation, which can make A. ochraceus protease a promising substitute for the snake activator in diagnostics for determining the content of factor X.
About the Authors
A. V. Orekhova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Biological Faculty.
Leninskiye gory 1—12, Moscow, 119234
A. A. Osmolovskiy
Lomonosov Moscow State University
Russian Federation
Russian Federation
Biological Faculty.
Leninskiye gory 1—12, Moscow, 119234
V. G. Kreyer
Lomonosov Moscow State University
Russian Federation
Russian Federation
Biological Faculty.
Leninskiye gory 1—12, Moscow, 119234N. A. Baranova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Biological Faculty.
Leninskiye gory 1—12, Moscow, 119234
N. S. Egorov
Lomonosov Moscow State University
Russian Federation
Russian Federation
International Biotechnology Center.
Leninskiye gory 1—12, Moscow, 119234
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Review
For citations:
Orekhova A.V., Osmolovskiy A.A., Kreyer V.G., Baranova N.A., Egorov N.S. Possibility of application of extracellular protease of micromycet Aspergillus ochraceus for determination of factor X content in human blood plasma. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2019;74(2):146–150. (In Russ.)
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