Mycology and Algology
The effect of mutations in genes encoding dehydrogenases and oxidases on the resistance of the cyanobacterium Synechocystis sp. PCC 6803 to oxidative stress inducer menadione was studied. Enhanced sensitivity to menadione was observed in the mutants carrying insertions in drgA gene encoding NAD(P)H: quinone-oxidoreductase (NQR) and in ndhB gene encoding the subunit of NDH-1 complex. Menadione resistance in the mutants lacking oxidases (Ox), succinate-dehydrogenase (SDH), and NDH-2 dehydrogenase was the same as in wild type cells. The additional mutation in drgA gene increased sensitivity to menadione in NDH-2 and Ox mutants. Double mutant lacking both of SDH and NQR was not viable. Expression of drgA gene decreased during cell incubation in the dark but increased in the presence of glucose both in the dark and in the light. Under photoautotrophic growth conditions dehydrogenase activity of the cells depends mainly of the NQR and NDH-1 functions. The rate of photosystem I reaction center (P700+) re-reduction after its oxidation with white light in the presence of DCMU increased in wild type and NDH-1 mutant after addition of menadione, whereas it decreased in NQR mutant. In the presence of menadiol the reduction of P700+ was accelerated in all strains studied. These data allow suggesting that NQR provides the defense of cyanobacterial cells from the toxic effect of menadione by its 2-electron reduction to menadiol. Increased sensitivity to menadione of NDH-1 mutant may results from the inhibition of respiration and cyclic electron transport around photosystem I.
In total, 2000 P. infestans isolates collected during 1988—2004 in different regions of Russia were tested for resistance to metalaxyl. In the majority of field populations frequency of resistant strains decreased after 1993—1994. Possibly it was connected with changes in potato industry in Russia: potato producing was concentrated in small private gardens. The part of resistant strains in populations from small private patches was less than at a big commercial potato fields. Small private gardens became the great source of sensitive genotypes. Last years the part of resistant strains in the majority of field populations was less than 30%. Small number of resistant strains in population occurs even if there was no treatment with metalaxyl—containing preparations during a long time. In some populations the frequency of resistant strains increased, it would be connected with treatments. A variation in the level of resistance to metalaxyl in one clonal linage is shown. Resistant strains occurred in potato leaves and tubers, in tomato leaves; they were rare in tomato fruits. Probably, the sensitive strains affecting fruits have selective advantage.
More than 370 strains from different regions were tested for resistance to dimethomorph. In several fields isolates were collected after treatment with dimethomorph—containing preparations. Resistant strains were not detected.
Physiology
Biophysics
Ecology
Microcolonies of bacteria living in a common fibrous matrix and dyes rust color with precipitating iron oxides are described. They are isomorphous (identical at sight) but represent different systematic groups. These microcolonies live both in fresh water and also present in soil moisture and activated sludge in airtanks. Their populations becomes exceedingly large, when biological transformation of organic substances due to the saturation of water with poorly oxidialing substances like soluble humus fractions, becomes disordered.
Data on the dynamic of the population of the microcolonies is used in new hydrobiological control processes of wastewater treatment and self-purification natural water — bioestimate. For distinguishing the microcolony the term siderotheca is suggest.
Fauna, Flora
In autumn period from March till May 1988 in Patagonium shelf and slope zones, the system of waters current modified and water temperature decreased. The biomass of seston and abundance of zooplancton decreased simultaneonsly, especially of subantarctic species Calanus simillimus. The zooplancton organisms started going down to deeper water layers. Antarctic species Rhincalanus gigas was common in upper layers in March. In May, R. gigas was rare and occured at larger depths. Subtropical zooplancton species dominated in shelf zone while subantarctic species dominated in the slope zone within the Folkiand current.
In May, the abundance of the shelf speciesin the slope zone increased because of wcakining of Falkland current and is dislocation to the east. The zooplancton biomass and abundance increased at the zone of water divergence in the cast and at the slope frontal zone in the west.