Рост мезенхимальных стволовых клеток на ориентированных микроструктурированных пленках и электроформованных скаффолдах

В работе получены пленки и скаффолды из поли-3-оксибутирата с различной микроструктурой тремя различными методами. Методом электроформования получены скаффолды с ориентированными и хаотично переплетенными волокнами различной толщины в зависимости от используемых параметров. Методами спин-коутинга и самоорганизации получены пленки с различной микроструктурой поверхности, в том числе с ориентированными элементами топографии. Показано, что микроструктура поверхности не влияет на рост мезенхимальных стволовых клеток в течение недели, однако оказывает влияние на морфологию прикрепленных клеток.

1. Werner M., Blanquer S.B.G., Haimi S., Korus G., Dunlop J.W.C., Duda G.N., Grijpma D.W., Petersen A. Surface curvature differentially regulates stem cell migration and differentiation via altered attachment morphology and nuclear deformation. Adv. Sci. 2017;4(2):1600347.

2. Van der Flier L.G., Clevers H. Stem cells, selfrenewal, and differentiation in the intestinal epithelium. Annu. Rev. Physiol. 2009;71:241–260.

3. Prittinen J., Zhou X., Bano F., Backman L., Danielson P. Microstructured collagen films for 3D corneal stroma modelling. Connect. Tissue Res. 2022;63(5):443–452.

4. Kim E.J., Boehm C.A., Mata A., Fleischman A.J., Muschler G.F., Roy S. Post microtextures accelerate cell proliferation and osteogenesis. Acta Biomater. 2010;6(1):160–169.

5. Koh L.B., Rodriguez I., Venkataraman S.S. The effect of topography of polymer surfaces on platelet adhesion. Biomaterials. 2010;31(7):1533–1545.

6. Li L., Chen C., Li J., Zhang A., Liu X., Xu B., Gao S., Jin G., Ma Z. Robust and hydrophilic polymeric films with honeycomb pattern and their cell scaffold applications. J. Mater. Chem. 2009;19(18):2789–2796.

7. Norrman K., Ghanbari-Siahkali A., Larsen N.B. 6 Studies of spin-coated polymer films. Annu. Rep. Prog. Chem., Sect. C: Phys. Chem. 2005;101:174–201.

8. Dong W., Zhou Y., Yan D., Mai Y., He L., Jin C. Honeycomb-structured microporous films made from hyperbranched polymers by the breath figure method. Langmuir. 2009;25(1):173–178.

9. Tokaruk W.A., Molteno T.C.A., Morris S.W. Bénard-Marangoni convection in two-layered liquids. Phys. Rev. Lett. 2000;84(16):3590–3593.

10. Wong K.H., Hernández-Guerrero M., Granville A.M., Davis T.P., Barner-Kowollik C., Stenzel M.H. Water-assisted formation of honeycomb structured porous films. J. Porous Mater. 2006;13(3):213–223.

11. Bondartsev A.P., Bonartseva G.A., Reshetov I.V., Shaitan K.V., Kirpichnikov M.P. Application of polyhydroxyalkanoates in medicine and the biological activity of natural poly(3-hydroxybutyrate). Acta Naturae. 2019;11(2):4–16.

12. Sunami H., Ito E., Tanaka M., Yamamoto S., Shimomura M. Effect of honeycomb film on protein adsorption, cell adhesion and proliferation. Colloids Surf. A Physicochem. Eng. Asp. 2006;284–285:548–551.

13. Xue J., Wu T., Dai Y., Xia Y. Electrospinning and electrospun nanofibers: Methods, materials, and applications. Chem. Rev. 2019;119(8):5298–5415.

14. Demina T.S., Bolbasov E.N., Peshkova M.A., Efremov Y.M., Bikmulina P.Y., Birdibekova A.V., Popyrina T.N., Kosheleva N.V., Tverdokhlebov S.I., Timashev P.S., Akopova T.A. Electrospinning vs. electro-assisted solution blow spinning for fabrication of fibrous scaffolds for tissue engineering. Polymers. 2022;14(23):5254.

15. Bonartsev A.P., Zharkova I.I., Voinova V.V., Kuznetsova E.S., Zhuikov V.A., Makhina T.K., Myshkina V.L., Potashnikova D.M., Chesnokova D.V., Khaydapova D.D., Bonartseva G.A., Shaitan K.V. Poly(3-hydroxybutyrate)/poly(ethylene glycol) scaffolds with different microstructure: the effect on growth of mesenchymal stem cells. 3 Biotech. 2018;8(8):328.

16. Pryadko A.S., Mukhortova Y.R., Chernozem R.V., et al. Electrospun magnetic composite poly-3-hydroxybutyrate/magnetite scaffolds for biomedical applications: Composition, structure, magnetic properties, and biological performance. ACS Appl. Bio Mater. 2022;5(8):3999–4019.

17. Kulikouskaya V.I., Nikalaichuk V.V., Bonartsev A.P., Akoulina E.A., Belishev N.V., Demianova I.V., Chesnokova D.V., Makhina T.K., Bonartseva G.A., Shaitan K.V., Hileuskaya K.S., Voinova V.V. Honeycomb-structured porous films from poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate): Physicochemical characterization and mesenchymal stem cells behavior. Polymers (Basel). 2022;14(13):2671.

18. Stalder A. F., Kulik G., Sage D., Barbieri L., Hoffmann P. A snake-based approach to accurate determination of both contact points and contact angles. Colloids Surf. A Physicochem. Eng. Asp. 2006;286(1–3):92–103.

19. Yusop N., Battersby P., Alraies A., Sloan A.J., Moseley R., Waddington R.J. Isolation and characterisation of mesenchymal stem cells from rat bone marrow and the endosteal niche: A comparative study. Stem Cells Int. 2018;2018:6869128.

20. Kumar P., Nagarajan A., Uchil P.D. Analysis of cell viability by the MTT assay. Cold Spring Harb. Protoc. 2018;2018(6):469–472.

21. Özen A., Gül Sancak İ., Tiryaki M., Ceylan A., Alparslan Pınarlı F., Delibaşı T. Mesenchymal stem cells (Mscs) in scanning electron microscopy (SEM) world. Niche. 2013;2:22–24.

22. Furukawa T., Sato H., Murakami R., Zhang J., Noda I., Ochiai S., Ozaki Y. Raman microspectroscopy study of structure, dispersibility, and crystallinity of poly (hydroxybutyrate)/poly (l-lactic acid) blends. Polymer. 2006;47(9):3132–3140.

23. Furukawa T., Sato H., Murakami R., Zhang J., Duan Y.X., Noda I., Shukichi O., Ozaki Y. Structure, dispersibility, and crystallinity of poly(hydroxybutyrate)/poly(l-lactic acid) blends studied by FT-IR microspectroscopy and differential scanning calorimetry. Macromolecules. 2005;38(15):6445–6454.