Injection of N-terminal fragment of nociceptin (FN) anxiolytic effect cockroach manifestation of anxiety in the test “open field” at a red light and illumination 6 W. An index of the level of anxiety was adopted intensity horizontal activity (HA) and number of changes of direction (CD) of insect. Introduction FN decreased the HA cockroaches at a red light to 48% (dose 100 mg/g) and 63% (dose 200 mg/g), and value of CD was 39% (100 mg) with respect to control. FN at a dose of 50 mg/g had no significant changes on the HA and CD. With increasing light stimulus value of HA rose to 230% and value CD rose to 192%, with respect to control. Injections of FN in this case, a natural increase of HA inhibition to 37% (100 mg/g) and to 50% (200 mg/g); value of CD reduced to 39% (100 mg/g) and to 68% (200 mg/g).

Effect of isotonic “Quinton Marine Plasma” (QMP) solution on the growth and “stationary phase aging” (accumulation of “age” changes in cultured cells during cell proliferation slowing down within a single passage and subsequent “aging” in the stationary phase of growth) of transformed Chinese hamster cells was studied. No positive effects of QMP on the studied viability indexes of the cultured cells were found in all the experiments. It is assumed that QMP, like many other potential anti-aging agents the authors studied recently (2,4-dinitrophenol in concentrations that provide mild uncoupling, the essential oil of oregano, hydrated C60-fullerene, etc.), can demonstrate its beneficial effect only at the level of the whole organism, triggering neurohumoral mechanisms that are not present in cytological model systems.

A simple method for purification of glucokinase from soluble fraction of rat liver has been developed which allows to obtain enzyme preparation with high specific activity and native cooperative characteristics practically lacking of contamination of other molecular forms of hexokinase. The dependence of enzyme activity upon glucose concentration shows pronounced sigmoid behavior: at 25°С and 2°С the Hillґs coefficients on whole range of substrate concentration are equal to 1,76 and 1,35, and S0,5 values for glucose are equal 6,9 mM and 20 mM, respectively. The role of kinetic mechanism of the reaction in the regulation of glucokinase activity in liver is discussed.

Analysis of the dynamics of the state of atmospheric air revealed adverse effects of the business activities of industrial productions on the environmental situation in the Novgorod region. Using correlation analysis, a relationship between the density of emissions of polluting substances into the atmosphere and the incidence of respiratory diseases was established. Air pollution accounted for 56,5% of disease cases in the population of municipalities and 6% of the children of Veliky Novgorod.

Species composition and frequency of occurrence of macrolichens on Betula ermanii and B. platyphylla were studied. Frequency of occurrence of the most species of these lichens was less than 10%. Frequency of occurrence of some species on different trunk levels was considerably different. Usually frequency of occurrence of macrolichens on B. ermanii was larger than on B. platyphylla. Distribution of 10 dominant macrolichen species on different trunk height was studied by One-factored ANOVA. Cladonia coniocraea, Parmeliopsis species and Vulpicida pinastri significantly occurs on the trunk bottoms. Cetraria sepincola had underdispersion on the two birches. Occurrence of Melanochalea species increases from bottom to branches on both birch species. Hypogymnia physodes, Parmelia squarrosa, P. sulcata certainly occur on the middle part of the trunks and branches only on B. ermanii and these lichens have underdispersion on B. platyphylla

The results of dependence of growth characteristics of strains Lactococcus lactis subsp. lactis 729 and TV2 isolated from milk and homemade curd the content in the agar medium peptone from casein as a main nitrogen source. Different composition on the level of enrichment of the agar medium was used for biomass growth strains in the study of growth characteristics in liquid media with the same composition. Compared the number of colony forming units (CFU), the rate of cell division (TDC) and the average hourly doubling time (AHDT) at two time points of cultivation: from 1,0 to 2,5 and from 2,5 to 3,5 hours. Liquid culture medium was maintained logarithmic growth of strain 729 in all variations on a time interval of cultivation to 3,5 hours with a maximum decrease in pH to 6,5. Maximum accumulation level of biomass (2,5 Ѕ 108 cells/ml) was observed when cultured in a pair of media containing meat extract, and the smallest AHDT (37,5 min) when moving biomass MA4 minimal medium (peptone from casein, and 0,4% lactose 0,4%) and rich in containing yeast extract liquid medium M7. Strain 729 in contrast to a strain incapable TB2 form colonies when the content of casein peptone agar medium is less than 0,4%. Reducing the amount of nitrogen source (peptone from casein from 1,0% to 0,4%) resulted in an increase in colony size strain TV2 from
2,85 mm2 to5mm2. The stimulatory effect of yeast extract in liquid media under these concentration ratios allowing cell division to increase the effectiveness while reducing the accumulation of lactic acid to achieve critical acidification (pH 5,5) blocks the transport of oligopeptides into a cell. Meat extract contributed bacteria adapt to the conditions of acidity and has a positive effect on cell division of strain 729 at a late stage of development of bacterial populations. Comparative small size of the colonies (area ~0,5 mm2 , diameter ~0,8 mm) can be a sign of strain as active acidifier.

Vibrational and acoustical signals in the species Trilophidia annulata (Thunb.) are described for the first time. Tremulatory vibration signals of the hind legs and tibia-tegminae clicks in both sexes as well as tegmino-fomoral stridulation males are described. The behavioral of the male near the female is studied. Oscillogramms are presented.

Greenish warblers try to keep the same weight level during the whole period of their staying in the breeding area. On the average, weight increases smoothly from the morning to the afternoon and evening. Weight changes follow diurnal changes of locomotor activity. In all stages fat reserves are significantly higher in the second part of the day. Changes of fat reserves follow the weight changes. Fat reserves changes are more significant than weight changes both at diurnal level and at different stages of life cycle. This finding supports the existence of double control system: the control of bird fly weight and that of energetic deposition weight. Greenish warblers depart from the breeding area with lower weight and fat depositions than those they arrive with.

Original data on leaf functional traits (leaf area and specific leaf area, SLA) in 80 vascular plant species in alpine plant communities of the Eastern Qinghai-Tibetan plateau (Sichuan, China) are represented. Mean SLA was 192 ± 6 cm2/g, and mean leaf area was 2,38 ± 0,23 cm2. The highest mean SLA was observed in loose-tussock grasses and the lowest SLA was in group of sedges and rushs, shrubs also had quite a low SLA. Among forbs erosulate plants had higher SLA than rosette forming species. Our results mostly confirm leaf trait patterns for other regions.