Physiology
In model experiments on the protozoa Spirostomum ambiguum Ehrbg stable dose-independent behavior change under the action of low radiation was studied. We have shown that even at a dose of 0,01 Gy, which is thousands times lower than predicted as lethal (1000 Gy), increases the pro- bability of spontaneous motor activity decreases. The negative effect is inherited by several genera- tions spirostom. Reduced motor activity is accompanied by morphologic violations of the protozoa, a manifestation of which is also dose-independent nature in a wide range of doses. It is discussion of biological testing of radioactive contamination of the environment using the criterion of change in motor activity of the protozoa and prospective studies of such effects.
Genetics
There are difficulties in studying of transgenic plants which associate with the instability of transformation, the inactivation of insertion and the derivation of somaclonal variants. This study describes the lines of tobacco plants, which were obtained by agrobacterial transformation and subsequent micropropagation in sterile conditions. Plants are characterized by dwarf length, increased proportion of sterile pollen grains and the lack of insertion which was detected before.
We characterized the floral structure of single mutants abr, ap3-1 and pi-1 and double mu
tants abr ap3-1 and abr pi-1 of Arabidopsis thaliana. The increasing of ap3-1 and pi-1 expressivity
on the background of abr mutation, which altered auxin transport, was detected. Unlike parental
mutants, which developed stamens in our experiment, the double mutants were not able to form
stamens in the basal flowers. It is assumed that the anomalies in the distribution of auxin in the
cells can disrupt the program of development of stamens, which turn on by alleles ap3-1 and pi-1,
retaining residual function when plants are grown under conditions of 21—24°C.
Ecology
Phytoplanklon was sampled in the Kandalaksha Bay of the White Sea and exposed in situ
14 days under two levels of light (E] > Ei) with the additions of ISO иМ of nitrogen as nitrate,
urea, ammonium and glycine. Every 3 days species composition, abundance and biomass of algae were estimated. The maximum biomass (Bnu4) of phvtoplankton depended on nitrogen substrates and light level. The differences in Bmax between phytoplankton assimilated different nitrogen substrates became more apparent under E>. Phytoplanklon assimilated nitrate, urea and ammonium had the higher Вщах tinder Ej. but algae grown with glycine had the higher B,luv under lb Although the values of Bmax differed, the structures of all assembles were similar except of that grown on ammonium under Ei. Competitive parameters of algae related to form of nitrogen and level of light. Comparison the data with results of analogous experiments in 2005 revealed that structure of assembles formed after nitrogen pulse depended on not only nitrogen substrates and light level but also on initial (before pulse) structure and nitrogen status of algae.
Mycology and Algology
Chaetomium is a fungus found on different substrates contained cellulose. Some species of the genera can destroy books, textile materials and valuable wood articles. Some of them are of great interest because of antagonistic and enzymic properties. Research of morphology and cultural characteristics of Chaetomium species is an interesting practical and theoretical problem. Growth and development colonies of C. globosum, C. funicola, C. elatum and C. spirale were studied at 17—20, 25, 27, 30 и 33—35°С, and on different carbon sources — glucose, sucrose, mannitol, lactose, starch and cellulose. Growth of colonies was optimal at 25—27°С for all investigated species and entirely week at 33—35°С. Growth dynamic and morphology of colonies, development of air mycelium and fruit bodies of different Chaetomium species significantly varied depend on carbon source. Comparison of craft-paper destruction was made for all investigated species and C. murorum. The fungi C. globosum, C. funicola and C. elatum were characterized by re- latively high destructive activities.
Co-cultivation of Pleurotus ostreatus with eight yeast species were investigated on water agar.
Special mycelial structures to contact with yeast cells were found in such cultures: nipple-like
appendages and coralloid hyphae. Three out of eight species, Hanseniaspora uvarum, Rhodotorula
minuta, and Saccharomyces cerevisiae were identified as trophic preferendum for P. ostreatus. These three yeast species were used for mushroom cultivation on sunflower seed’s peel. The biomass of fruiting bodies increased on 52,8—75,7% with the H. uvarum and S. cerevisiae suspension presence in the substrate.
Fauna, Flora
Crested auklet — unique species of Alcidae, how display complex social behavior and breeds in dense colonies included up to million individuals. Here we studied individual differences in trumpet calls — loud vocalization, used by auklets as advertising display. In 2008 and 2009 we recorded 231 calls, from 24 marked birds from Talan island (Sea of Okhotsk). The discriminant analysis, based on 7 parameters of introduction, showed 84,8%; and based on 7 parameters of main part — 82,3% of correct assignment. Both values are significantly higher the random value. Our data suggest that trumpet calls of crested auklets carry strong individually specific features. Even introduction or main part is potentially sufficient for individual recognition of calling bird. We suppose that this superfluity of individually specific information is one of possible adaptation for communication in noisy environment of dense colonies: anklets need to hear only the small part ofthe trumpet cafor undbubtedTidentification of calling bird.